24.2 What is Solid Phase Extraction?
Description
This article is from the Chemistry FAQ, by Bruce Hamilton B.Hamilton@irl.cri.nz with numerous contributions by others.
24.2 What is Solid Phase Extraction?
Solid Phase Extraction (SPE) is an alternative to liquid/liquid extraction,
and has become the method of choice for the separation and purification of
a wide range of samples in the laboratory. The sample is usually dissolved
in an appropriate solvent and passed through a small bed of adsorbent of
very consistent particle size and shape to maximise separation efficiency.
The compounds are eluted with step changes of small volumes of solvents.
The major advantage is that solvent volumes are greatly reduced. There is
a newer, modified technique that is used in analytical laboratories, called
Solid Phase Micro Extraction. This immerses a fused silica fibre coated with
a stationary phase into the sample solution for several minutes, The analytes
adsorb onto the stationary phase, which is subsequently pushed into a hot GC
injector to rapidly desorb the sample for analysis.
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