This article is from the Model Trains FAQ, by Christopher D Coleman with numerous contributions by others.
Yes, as long as you use similar locomotives. What I mean by this is
some locos use can motors, some use "universal" motors in addition to
various gearing ratios. To test two locos for compatibility set them
on the track, uncoupled and unloaded and run them in the same
direction. If the separation between them rapidly increases or
decreases their natural speeds are too far apart and they will fight
each other if coupled together. You MUST lock out your sequencers when
you doublehead since a momentary power loss may sequence one loco and
not another (unless you have electronic ones which suppress this
problem). Mid-train helpers are also possible but placing requires
skill and practice. Rear helpers are not recommended.
 
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